2x xt (Bio-Rad)
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2x xt
2x Xt, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2x+xt/XT+Sample+Buffer/bio_rxiv__64898__2026__03__23__713706-260-12-16
Average 96 stars, based on 453 article reviews
2x Xt, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2x+xt/XT+Sample+Buffer/bio_rxiv__64898__2026__03__23__713706-260-12-16
Average 96 stars, based on 453 article reviews
2x xt - by Bioz Stars,
2026-09
96/100 stars
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Incubation:Article Title: The DNA-Binding Domain Mediates both Nuclear and Cytosolic Functions of p53 Article Snippet: Cells were lysed in 0.4 mL ice-cold buffer containing 50 mM tris, 150 mM NaCl, 1 mM EDTA, 0.5 % NP40, pH 7.4 supplemented with protease inhibitor tablets (Roche). .. Cell lysates were incubated with 20 μL Protein A/G beads (Santa Cruz Biotechnology) supplemented with 1 μg anti-p53 antibody (clone DO-7, Santa Cruz Biotechnology at 1:1000 dilution – for H1299 cells transfected with p53 constructs), or 1 μg anti GFP antibody (mixed 7.1 and 13.1 clones, Roche at 1:2000 dilution – for HCT116 cells transfected with Cerulean BCL-xL constructs) for 2 hours at 4 degrees, washed 4 times with cold lysis buffer and eluted by boiling in 50 μL Transfection:Article Title: The DNA-Binding Domain Mediates both Nuclear and Cytosolic Functions of p53 Article Snippet: Cells were lysed in 0.4 mL ice-cold buffer containing 50 mM tris, 150 mM NaCl, 1 mM EDTA, 0.5 % NP40, pH 7.4 supplemented with protease inhibitor tablets (Roche). .. Cell lysates were incubated with 20 μL Protein A/G beads (Santa Cruz Biotechnology) supplemented with 1 μg anti-p53 antibody (clone DO-7, Santa Cruz Biotechnology at 1:1000 dilution – for H1299 cells transfected with p53 constructs), or 1 μg anti GFP antibody (mixed 7.1 and 13.1 clones, Roche at 1:2000 dilution – for HCT116 cells transfected with Cerulean BCL-xL constructs) for 2 hours at 4 degrees, washed 4 times with cold lysis buffer and eluted by boiling in 50 μL Construct:Article Title: The DNA-Binding Domain Mediates both Nuclear and Cytosolic Functions of p53 Article Snippet: Cells were lysed in 0.4 mL ice-cold buffer containing 50 mM tris, 150 mM NaCl, 1 mM EDTA, 0.5 % NP40, pH 7.4 supplemented with protease inhibitor tablets (Roche). .. Cell lysates were incubated with 20 μL Protein A/G beads (Santa Cruz Biotechnology) supplemented with 1 μg anti-p53 antibody (clone DO-7, Santa Cruz Biotechnology at 1:1000 dilution – for H1299 cells transfected with p53 constructs), or 1 μg anti GFP antibody (mixed 7.1 and 13.1 clones, Roche at 1:2000 dilution – for HCT116 cells transfected with Cerulean BCL-xL constructs) for 2 hours at 4 degrees, washed 4 times with cold lysis buffer and eluted by boiling in 50 μL Clone Assay:Article Title: The DNA-Binding Domain Mediates both Nuclear and Cytosolic Functions of p53 Article Snippet: Cells were lysed in 0.4 mL ice-cold buffer containing 50 mM tris, 150 mM NaCl, 1 mM EDTA, 0.5 % NP40, pH 7.4 supplemented with protease inhibitor tablets (Roche). .. Cell lysates were incubated with 20 μL Protein A/G beads (Santa Cruz Biotechnology) supplemented with 1 μg anti-p53 antibody (clone DO-7, Santa Cruz Biotechnology at 1:1000 dilution – for H1299 cells transfected with p53 constructs), or 1 μg anti GFP antibody (mixed 7.1 and 13.1 clones, Roche at 1:2000 dilution – for HCT116 cells transfected with Cerulean BCL-xL constructs) for 2 hours at 4 degrees, washed 4 times with cold lysis buffer and eluted by boiling in 50 μL Lysis:Article Title: The DNA-Binding Domain Mediates both Nuclear and Cytosolic Functions of p53 Article Snippet: Cells were lysed in 0.4 mL ice-cold buffer containing 50 mM tris, 150 mM NaCl, 1 mM EDTA, 0.5 % NP40, pH 7.4 supplemented with protease inhibitor tablets (Roche). .. Cell lysates were incubated with 20 μL Protein A/G beads (Santa Cruz Biotechnology) supplemented with 1 μg anti-p53 antibody (clone DO-7, Santa Cruz Biotechnology at 1:1000 dilution – for H1299 cells transfected with p53 constructs), or 1 μg anti GFP antibody (mixed 7.1 and 13.1 clones, Roche at 1:2000 dilution – for HCT116 cells transfected with Cerulean BCL-xL constructs) for 2 hours at 4 degrees, washed 4 times with cold lysis buffer and eluted by boiling in 50 μL |